Centrifuges

Important issues to notice when performing centrifugal separations: Centrifugation data should be stated either in units of Relative Centrifugal Force (RCF) or gravities (g), or the data should be stated so RCF can be calculated using revolutions per minute (RPM) and the dimensions of the centrifuge rotor. Centrifugation at the same RPM and duration will exert different centrifugal forces on samples if centrifuge rotors having different radius sizes, bucket types or bucket sizes are used. Different centrifugal forces produce different sample separation rates at the same RPM and duration. In order to reproduce the experimental conditions when a different type or make of rotor is to be used, it is essential to know the precise dimensions and characteristics of each rotor. If the RPM and rotor radius are known, a monogram chart can be used to determine the RCF. RCF (or g) can be calculated by using the following formula (Rickwood, 1984, p. 288):


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